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Label-free electrochemical aptasensing platform based on mesoporous silica thin film for the detection of prostate specific antigen.
- Source :
-
Sensors & Actuators B: Chemical . Feb2018 Part 1, Vol. 255, p309-315. 7p. - Publication Year :
- 2018
-
Abstract
- We report the development of a novel label-free electrochemical aptasensing platform to detect prostate specific antigen (PSA) based on mesoporous silica thin film-coated gold electrodes as a sensing interface. The aptasensing approach relies on hindering the diffusion of [Fe(CN) 6 ] 3/4− redox probe through the nanochannels of the mesoporous film gated by the recognition of the target biomarker by its anti-PSA specific DNA aptamer covalently attachment onto the outer surface of the silica nanopores. The aptasensing platform was found to be very sensitive to PSA in the range from 1 to 300 ng mL −1 with a detection limit of 280 pg mL −1 . The aptasensor also showed high specificity, reproducibility and storage stability, and was successfully validated in spiked artificial urine samples and in blood serum from a prostate cancer-free male patient. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 255
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 125806341
- Full Text :
- https://doi.org/10.1016/j.snb.2017.08.045