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A fast-response two-photon fluorescent probe for imaging endogenous H2O2 in living cells and tissues.

Authors :
Lu, Yanan
Shi, Xiaomin
Fan, Wenlong
Black, Cory A.
Lu, Zhengliang
Fan, Chunhua
Source :
Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy. Feb2018, Vol. 190, p353-359. 7p.
Publication Year :
2018

Abstract

As a second messenger, hydrogen peroxide plays significant roles in numerous physiological and pathological processes and is related to various diseases including inflammatory disease, diabetes, neurodegenerative disorders, cardiovascular disease and Alzheimer's disease. Two-photon (TP) fluorescent probes reported for the detection of endogenous H 2 O 2 are rare and most have drawbacks such as slow response and low sensitivity. In this report, we demonstrate a simple H 2 O 2 -specific TP fluorescent probe ( TX-HP ) containing a two-photon dye 6-hydroxy-2,3,4,4a-tetrahydro-1H-xanthen-1-one ( TX ) on the modulation of the ICT process. The probe exhibits a rapid fluorescent response to H 2 O 2 in 9 min with both high sensitivity and selectivity. The probe can detect exogenous H 2 O 2 in living cells. Furthermore, the probe is successfully utilized for imaging H 2 O 2 in liver tissues. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13861425
Volume :
190
Database :
Academic Search Index
Journal :
Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy
Publication Type :
Academic Journal
Accession number :
125968919
Full Text :
https://doi.org/10.1016/j.saa.2017.09.034