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Scattering measurement of single particle for highly sensitive homogeneous detection of DNA in serum.
- Source :
-
Talanta . Feb2018, Vol. 178, p545-551. 7p. - Publication Year :
- 2018
-
Abstract
- A highly sensitive homogeneous method for DNA detection has been developed. The system relies on two kinds of gold nanorod (AuNR) probes with complementary DNA sequences to the target DNA. In the presence of the target DNA, two kinds of AuNR probes are assembling into dimers or small aggregates. The target-induced AuNR aggregate has higher scattering intensity than that of a single AuNR because of the plasmonic coupling effect. Dark field microscopy was utilized to image the single particle and measure its scattering intensity. We wrote our own Matlab code and used it to extract the scattering signal of all particles. Difference in distribution of scattering intensity between the single AuNR and its aggregate provides a quantitative basis for the detection of target DNA. A linear dynamic range spanning from 0.1 pM to 1 nM and a detection limit of ~ 30 fM were achieved for the detection of DNA in serum sample. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NUCLEOTIDE sequencing
*SCATTERING (Physics)
*PARTICLE detectors
*SERUM
*DIMERS
Subjects
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 178
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 126120365
- Full Text :
- https://doi.org/10.1016/j.talanta.2017.09.052