A facile fluorescent probe with a large Stokes shift for sequentially detecting copper and sulfide in 100% aqueous solution and imaging them in living cells.

The selective detection of Cu 2+ and S 2− in pure water systems is very challenging. To address this problem, we here present a novel fluorescent probe CF536 for the reversible detection of Cu 2+ and S 2− in a 100% aqueous solution via a displacement approach based on fluorescence quenching and the ESIPT mechanism, and characterized by IR, NMR, HRMS and X-ray analysis. The introduction of Cu 2+ (1.0 equiv.) to the aqueous solution of CF536 led to quenching of the intense green fluorescence within 2 min. On the addition of S 2− (3.0 equiv.), the emission of the ESIPT probe soon recovered from the CF536 -Cu 2+ system due to the abolishment of paramagnetic Cu 2+ . The fluorescence “ON-OFF-ON” cycle can be repeated a minimum of 5 times by the alternate addition of Cu 2+ and S 2− , implying that CF536 is a renewable dual-functional probe. The probe displayed high selectivity, a rapid response time, excellent water solubility due to introduction of the extremely hydrophilic trihydroxyl group and outstanding anti-interference ability for its large Stokes shift of 101 nm (λ abs = 435 nm, λ em = 536 nm), thus making the method highly suitable for bioimaging. The probe CF536 was successfully used for fluorescent imaging of copper and hydrogen sulfide in living cells. [ABSTRACT FROM AUTHOR]