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Chemical reprogramming of mouse embryonic and adult fibroblast into endoderm lineage.
- Source :
-
Journal of Biological Chemistry . 11/17/2017, Vol. 292 Issue 46, p19122-19132. 11p. - Publication Year :
- 2017
-
Abstract
- We report here an approach to redirecting somatic cell fate under chemically defined conditions without transcription factors. We start by converting mouse embryonic fibroblasts to epithelial-like cells with chemicals and growth factors. Subsequent cell fate mapping reveals a robust induction of SOX17 in the resulting epithelial-like cells that can be further reprogrammed to endodermal progenitor cells. Interestingly, these cells can self-renew in vitro and further differentiate into albumin- producing hepatocytes that can rescue mice from acute liver injury. Our results demonstrate a rational approach to convert mouse embryonic fibroblasts to hepatocytes and suggest that this mechanism-driven approach may be generalized for other cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ENDODERM
*CELL determination
*SOMATIC cells
*TRANSCRIPTION factors
*LABORATORY mice
Subjects
Details
- Language :
- English
- ISSN :
- 00219258
- Volume :
- 292
- Issue :
- 46
- Database :
- Academic Search Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 126370906
- Full Text :
- https://doi.org/10.1074/jbc.M117.812537