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Overexpression of SbMyb60 in Sorghum bicolor impacts both primary and secondary metabolism.
- Source :
-
New Phytologist . Jan2018, Vol. 217 Issue 1, p82-104. 23p. - Publication Year :
- 2018
-
Abstract
- Few transcription factors have been identified in C4 grasses that either positively or negatively regulate monolignol biosynthesis., Previously, the overexpression of SbMyb60 in sorghum ( Sorghum bicolor) has been shown to induce monolignol biosynthesis, which leads to elevated lignin deposition and altered cell wall composition. To determine how SbMyb60 overexpression impacts other metabolic pathways, RNA-Seq and metabolite profiling were performed on stalks and leaves., 35S::SbMyb60 was associated with the transcriptional activation of genes involved in aromatic amino acid, S-adenosyl methionine ( SAM) and folate biosynthetic pathways. The high coexpression values between SbMyb60 and genes assigned to these pathways indicate that SbMyb60 may directly induce their expression. In addition, 35S::SbMyb60 altered the expression of genes involved in nitrogen (N) assimilation and carbon (C) metabolism, which may redirect C and N towards monolignol biosynthesis. Genes linked to UDP-sugar biosynthesis and cellulose synthesis were also induced, which is consistent with the observed increase in cellulose deposition in the internodes of 35S::SbMyb60 plants. However, SbMyb60 showed low coexpression values with these genes and is not likely to be a direct regulator of cell wall polysaccharide biosynthesis., These findings indicate that SbMyb60 can activate pathways beyond monolignol biosynthesis, including those that synthesize the substrates and cofactors required for lignin biosynthesis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0028646X
- Volume :
- 217
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- New Phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 126440198
- Full Text :
- https://doi.org/10.1111/nph.14815