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Involvement of phosphatidylinositol 3-kinase and insulin-like growth factor-I in YXLST-mediated neuroprotection
- Source :
-
Brain Research . May2004, Vol. 1006 Issue 2, p198. 9p. - Publication Year :
- 2004
-
Abstract
- In the present study, we examine the neuroprotective role of the external Qi of YXLST in cultured retinal neurons. Primary retinal neuronal cultures were grown from retinas of 0–2-day-old Sprague–Dawley rats. Cultures were treated directly with external Qi of YXLST 30 min prior to H2O2 exposure in most experiments. Cell viability was measured by 3,(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay. Apoptotic cell death was evaluated by the TdT-mediated digoxigenin-dUTP nick-end labeling TUNEL assay, and by DNA laddering analysis. Northern blot analysis was performed to examine the level of insulin-like growth factor-I (IGF-I) gene expression. Phosphatidylinositol 3-kinase (PI3K) assay was performed to study the PI3K activity. The results showed that treatment of external Qi of YXLST significantly attenuated neuronal death that was induced by 24-h exposure to hydrogen peroxide, and greatly inhibited hydrogen peroxide-induced apoptosis. External Qi of YXLST also upregulated IGF-I gene expression and increased PI3K activity. These observations indicate that external Qi-mediated IGF-I expression and PI3K signaling could be one of the mechanisms in neuroprotection by YXLST. [Copyright &y& Elsevier]
- Subjects :
- *PHOSPHOINOSITIDES
*GROWTH factors
*PROTEIN kinases
*NEURONS
Subjects
Details
- Language :
- English
- ISSN :
- 00068993
- Volume :
- 1006
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Brain Research
- Publication Type :
- Academic Journal
- Accession number :
- 12651630
- Full Text :
- https://doi.org/10.1016/j.brainres.2004.01.068