Cultivos a cielo abierto de las cianobacterias Nostoc LAUN0015 y Anabaena MOF015 para la producción de biomasa enriquecida. Pruebas piloto para cultivos masivos.

The growing world food demand has led to the search for new sources of highly nutritious biomass. Cyanobacteria can be used not only as a source of compounds of biotechnological interest, but also as food for animals and humans, because of their high nutritional quality. The aim of this study was to evaluate the biomass production of nitrogen fixing filamentous cyanobacteria Nostoc LAUN0015 and Anabaena MOF015 outdoors at two culture volumes: 50 and 100 L using BG11 with no nitrogen as culture medium without nitrogen. Growth was followed by turbidity and determination of pigments until reaching stationary phase of growth. The Biomass was harvested, dried and used for the determination of dry mass of protein, carbohydrates and phycohiliprotrin total lipids. Anabaena MOF015 produced the highest values of dry mass, chlorophyll, phycocyanin, phycoerythrin, proteins and lipids with 3.85 ±0.41 g L-1, 3.76 ±0.51, 6.16 ±0.85, 2.79 ±0.25, 250.31 ±10.01 and 20.11 ±1.01 μg mL-1 respectively in a volume of 50 L culture. Nostoc LAUN0015 reached the maximum production of carbohydrates of 754.17 ±79.86 μg mL-1. Anabaena MOF015 is prominent for further growth and production of dry biomass and metabolites (p<0.05) in diazotrophic conditions, which means more economical culture systems with great biotechnological value. [ABSTRACT FROM AUTHOR]