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A turn-on fluorescence aptasensor based on carbon dots for sensitive detection of adenosine.
- Source :
-
New Journal of Chemistry . 9/7/2017, Vol. 41 Issue 17, p9230-9235. 6p. - Publication Year :
- 2017
-
Abstract
- In this paper, a novel turn-on fluorescence aptasensor has been designed for adenosine detection based on fluorescence resonance energy transfer (FRET) from single-stranded DNA labeled carbon dots (ssDNA-CDs) to aptamer modified gold nanoparticles (aptamer-AuNPs). In the absence of adenosine, the fluorescence of ssDNA-CDs was quenched by aptamer-AuNPs via the formation of an aptamer–ssDNA duplex. The introduced adenosine competed to displace ssDNA-CDs by specifically binding to the aptamer, resulting in the recovery of the quenched fluorescence of ssDNA-CDs. Under optimized conditions, the increase in fluorescence intensity was proportional to the concentration of adenosine with a linear range of 10–500 nM and allowed a limit of detection as low as 4.2 nM. In addition, this method was successfully applied to adenosine determination in human serum samples with satisfactory results. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ADENOSINES
*FLUORESCENCE resonance energy transfer
*SINGLE-stranded DNA
Subjects
Details
- Language :
- English
- ISSN :
- 11440546
- Volume :
- 41
- Issue :
- 17
- Database :
- Academic Search Index
- Journal :
- New Journal of Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 126779490
- Full Text :
- https://doi.org/10.1039/c7nj02384g