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Functional analyses of the CIF1-CIF2 complex in trypanosomes identify the structural motifs required for cytokinesis.
- Source :
-
Journal of Cell Science . 12/15/2017, Vol. 130 Issue 24, p4108-4119. 12p. - Publication Year :
- 2017
-
Abstract
- Cytokinesis in trypanosomes occurs uni-directionally along the longitudinal axis from the cell anterior towards the cell posterior and requires a trypanosome-specific CIF1-CIF2 protein complex. However, little is known about the contribution of the structural motifs in CIF1 and CIF2 to complex assembly and cytokinesis. Here, we demonstrate that the two zinc-finger motifs but not the coiled-coil motif in CIF1 are required for interaction with the EF-handmotifs in CIF2.We further show that localization of CIF1 depends on the coiled-coil motif and the first zinc-finger motif and that localization of CIF2 depends on the EF-hand motifs. Deletion of the coiled-coil motif and mutation of either zinc-finger motif in CIF1 disrupts cytokinesis. Furthermore, mutation of either zinc-finger motif in CIF1 mislocalizes CIF2 to the cytosol and destabilizes CIF2, whereas deletion of the coiled-coil motif in CIF1 spreads CIF2 over to the new flagellum attachment zone and stabilizes CIF2. Together, these results uncover the requirement of the coiled-coil and zinc-fingermotifs for CIF1 function in cytokinesis and for CIF2 localization and stability, providing structural insights into the functional interplay between the two cytokinesis regulators. [ABSTRACT FROM AUTHOR]
- Subjects :
- *TRYPANOSOMA
*CYTOKINESIS
*PROTOZOAN proteins
Subjects
Details
- Language :
- English
- ISSN :
- 00219533
- Volume :
- 130
- Issue :
- 24
- Database :
- Academic Search Index
- Journal :
- Journal of Cell Science
- Publication Type :
- Academic Journal
- Accession number :
- 126895565
- Full Text :
- https://doi.org/10.1242/jcs.207134