五子衍宗丸对幼年大鼠睾丸支持细胞增殖的影响及机制.

Objective To discuss the effect and mechanism of Wuzi Yanzong Wan (Five Seeds for Multiplying Pills) in the proliferation of Sertoli cells in infant rats. Methods The Sertoli cells were separated from male SD rats (20 d old) and cultured, and then randomly divided into normal group, low-dose Wuzi Yanzong Wan group (5 g/L, low-dose group), mid-dose Wuzi Yanzong Wan group (10 g/L, mid-dose group) and high-dose Wuzi Yanzong Wan group (15 g/L, high-dose group). The changes of optical density (OD) of Sertoli cells were detected by using methyl thiazolyl tetrazolium test (MTT) in all groups. The expression of Ki67 positive granules in Sertoli cells were detected by using immunohistochemistry technique in all groups. The expressions of Akt and p-Akt in Sertoli cells were detected by using Western blot method. Results Compared with normal group, the proliferation of Sertoli cells treated with Wuzi Yanzong Wan was promoted significantly (P<0.05 or P<0.01). The expression of Ki67 positive granules increased significantly (P<0.05 or P<0.01), and relative expression of p-Akt increased significantly (P<0.05 or P<0.01). Conclusion Wuzi Yanzong Wan can possibly promote the proliferation of Sertoli cells in infant rats through activating Akt signal transduction pathway and up-regulating p-Akt expression. [ABSTRACT FROM AUTHOR]