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The actin-organizing formin protein Fhod3 is required for postnatal development and functional maintenance of the adult heart in mice.

Authors :
Tomoki Ushijima
Noriko Fujimoto
Sho Matsuyama
Meikun Kan-o
Hiroshi Kiyonari
Go Shioi
Yohko Kage
Sho Yamasaki
Ryu Takeya
Hideki Sumimoto
Source :
Journal of Biological Chemistry. 1/5/2018, Vol. 293 Issue 1, p148-162. 15p.
Publication Year :
2018

Abstract

Cardiac development and function require actin-myosin interactions in the sarcomere, a highly organized contractile structure. Sarcomere assembly mediated by formin homology 2 domain-containing 3 (Fhod3), a member of formins that directs formation of straight actin filaments, is essential for embryonic cardiogenesis. However, the role of Fhod3 in the neonatal and adult stages has remained unknown. Here, we generated floxed Fhod3 mice to bypass the embryonic lethality of an Fhod3 knockout (KO). Perinatal KO of Fhod3 in the heart caused juvenile lethality at around day 10 after birth with enlarged hearts composed of severely impaired myofibrils, indicating that Fhod3 is crucial for postnatal heart development. Tamoxifen-induced conditional KO of Fhod3 in the adult heart neither led to lethal effects nor did it affect sarcomere structure and localization of sarcomere components. However, adult Fhod3-deleted mice exhibited a slight cardiomegaly and mild impairment of cardiac function, conditions that were sustained over 1 year without compensation during aging. In addition to these age-related changes, systemic stimulation with the α1-adrenergic receptor agonist phenylephrine, which induces sustained hypertension and hypertrophy development, induced expression of fetal cardiac genes that was more pronounced in adult Fhod3-deleted mice than in the control mice, suggesting that Fhod3 modulates hypertrophic changes in the adult heart. We conclude that Fhod3 plays a crucial role in both postnatal cardiac development and functional maintenance of the adult heart. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219258
Volume :
293
Issue :
1
Database :
Academic Search Index
Journal :
Journal of Biological Chemistry
Publication Type :
Academic Journal
Accession number :
127182938
Full Text :
https://doi.org/10.1074/jbc.M117.813931