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Mechanism of Chorismate Synthase.
- Source :
-
Journal of Biological Chemistry . 3/5/2004, Vol. 279 Issue 10, p9451-9461. 11p. 1 Color Photograph, 3 Diagrams, 1 Chart, 8 Graphs. - Publication Year :
- 2004
-
Abstract
- Chorismate synthase catalyzes the last step in the common shikimate pathway leading to aromatic compounds such as the aromatic amino acids. The reaction consists of the 1,4-anti-elimination of the 3-phosphate group and the C-(6proR) hydrogen from 5-enolpyruvylshikimate 3-phosphate to yield chorismate. Although this reaction does not involve a net redox change, the enzyme has an absolute requirement for reduced flavin mononucleotide, which is not consumed during the reaction. Two invariant histidine residues are found in the active site of the enzyme: His17 and His106. Using sitedirected mutagenesis, both histidines were replaced by alanine, reducing the activity 10- and 20-fold in the H106A and H17A mutant protein, respectively. Based on the characterization of the two single mutant proteins, it is proposed that His106 serves to protonate the monoanionic reduced FMN, whereas His17 protonates the leaving phosphate group of the substrate. An enzymatic reaction mechanism in keeping with the experimental results is presented. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219258
- Volume :
- 279
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12732412
- Full Text :
- https://doi.org/10.1074/jbc.M312471200