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Lidocaine increases phosphorylation of focal adhesion kinase in rat hippocampal slices
- Source :
-
European Journal of Pharmacology . Apr2004, Vol. 489 Issue 1/2, p55. 4p. - Publication Year :
- 2004
-
Abstract
- We examined the effect of lidocaine on phosphorylation of the tyrosine kinase focal adhesion kinase (PP125FAK) in rat hippocampal slices by immunoblotting with both antiphosphotyrosine and specific anti-PP125FAK antibodies in the presence of tetrodotoxin (1 μM). Lidocaine induced a concentration-related increase in tyrosine phosphorylation of the 125-kDa band corresponding to PP125FAK phosphorylation (EC50 value=0.39±0.09 μM, maximal effect=169±28% of control, P<0.001). This effect was sensitive to neither the N-methyl-d-aspartate (NMDA) receptor antagonist dizocilpine (MK 801, 10 μM) nor the inhibitor of the ryanodine receptor dantrolene (30 μM). In contrast, it was completely blocked by the protein kinase C (PKC) inhibitors chelerythrin, bisindolylmaleimide I (GF 109203X) and bisindolylmaleimide IX (RO-318220, 10 μM). We conclude that lidocaine increases phosphorylation of the tyrosine kinase PP125FAK in the rat hippocampus by a tetrotoxin (TTX)-insensitive mechanism which involves activation of PKC. [Copyright &y& Elsevier]
- Subjects :
- *FOCAL adhesion kinase
*LIDOCAINE
*PROTEIN kinase C
*HIPPOCAMPUS (Brain)
Subjects
Details
- Language :
- English
- ISSN :
- 00142999
- Volume :
- 489
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- European Journal of Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 12746789
- Full Text :
- https://doi.org/10.1016/j.ejphar.2004.02.012