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Molecular design for recombinant adeno-associated virus (rAAV) vector production.
- Source :
-
Applied Microbiology & Biotechnology . Feb2018, Vol. 102 Issue 3, p1045-1054. 10p. - Publication Year :
- 2018
-
Abstract
- Recombinant adeno-associated virus (rAAV) vectors are increasingly popular tools for gene therapy applications. Their non-pathogenic status, low inflammatory potential, availability of viral serotypes with different tissue tropisms, and prospective long-lasting gene expression are important attributes that make rAAVs safe and efficient therapeutic options. Over the last three decades, several groups have engineered recombinant AAV-producing platforms, yielding high titers of transducing vector particles. Current specific productivity yields from different platforms range from 10 to 10 vector genomes (vg) per cell, and there is an ongoing effort to improve vector yields in order to satisfy high product demands required for clinical trials and future commercialization. Crucial aspects of vector production include the molecular design of the rAAV-producing host cell line along with the design of AAV genes, promoters, and regulatory elements. Appropriately, configuring and balancing the expression of these elements not only contributes toward high productivity, it also improves process robustness and product quality. In this mini-review, the rational design of rAAV-producing expression systems is discussed, with special attention to molecular strategies that contribute to high-yielding, biomanufacturing-amenable rAAV production processes. Details on molecular optimization from four rAAV expression systems are covered: adenovirus, herpesvirus, and baculovirus complementation systems, as well as a recently explored yeast expression system. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ADENO-associated virus
*GENE therapy
*GENETIC vectors
*HERPESVIRUSES
*BACULOVIRUSES
Subjects
Details
- Language :
- English
- ISSN :
- 01757598
- Volume :
- 102
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Applied Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 127474562
- Full Text :
- https://doi.org/10.1007/s00253-017-8670-1