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Characterization and identification of in vitro metabolites of (-)-epicatechin using ultra-high performance liquid chromatography-mass spectrometry.
- Source :
-
Tropical Journal of Pharmaceutical Research . Dec2017, Vol. 16 Issue 12, p2985-2990. 6p. - Publication Year :
- 2017
-
Abstract
- Purpose: To characterize and identify metabolites of (-)-epicatechin in microsomal fraction of rat hepatocytes (MFRHs). Methods: A single incubation of (-)-epicatechin (1 mL, 50 ìg/mL) in MFRH (0.5 mg/mL) was used for the generation of metabolites. Thereafter, the sample was subjected to protein precipitation prior to analysis with ultra-high performance liquid chromatography coupled to linear ion-trap orbitrap mass spectrometry (UHPLC-LTQ-Orbitap MS). Results: Nine metabolites of (-)-epicatechin were characterized on the basis of high resolution mass measurement, MS spectra and literature data. Based on their structures, the major metabolic routes of (-)-epicatechin in MFRHs were identified as hydroxylation, dihydroxylation and glycosylation. Conclusion: This is the first report on metabolites of (-)-epicatechin in MFRHs, and it is helpful in gaining deeper insight into the metabolism of (-)-epicatechin in vivo. The results will also provide guidance in research on the pharmacokinetics of new drugs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15965996
- Volume :
- 16
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Tropical Journal of Pharmaceutical Research
- Publication Type :
- Academic Journal
- Accession number :
- 127497498
- Full Text :
- https://doi.org/10.4314/tjpr.v16i12.24