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Detection of beef adulteration by mitochondrial D-loop based species-specific polymerase chain reaction.
- Source :
-
International Journal of Food Properties . 2017 Supplement, Vol. 20, p2264-2271. 8p. 3 Black and White Photographs, 1 Diagram, 1 Chart. - Publication Year :
- 2017
-
Abstract
- A species-specific polymerase chain reaction (PCR) was developed for the identification of beef that is cattle (Bos indicus) meat by targeting mitochondrial D-loop region. A pair of beef-specific primers was designed to amplify conserved region of D-loop and possibility of cross-amplification was ruled by taking into account other species comprising of mammals, birds, rodents and fish. The amplified PCR product of 381 bp was evident in raw (n = 20), cooked (60, 80 and 100°C), autoclaved (121°C) and micro-oven processed beef samples. Sensitivity of PCR assay was at 0.1% for detection of beef adulteration and the limit of detection (LOD) of beef DNA was 0.1 picograms (pg). Newly developed beef-specific PCR assay was rapid and sensitive and hence could be applied for the detection of adulteration of meat. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10942912
- Volume :
- 20
- Database :
- Academic Search Index
- Journal :
- International Journal of Food Properties
- Publication Type :
- Academic Journal
- Accession number :
- 127560928
- Full Text :
- https://doi.org/10.1080/10942912.2017.1369103