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Nucleotide variability in Beta 2 Microglobulin (β2M) gene and its association with colostral IgG levels in buffaloes (Bubalus bubalis).
- Source :
-
Indian Journal of Animal Research . Jan2018, Vol. 52 Issue 1, p51-55. 5p. - Publication Year :
- 2018
-
Abstract
- The present study was taken up to find out Beta 2 Microglobulin (β2M) gene variants and their association with IgG concentrations in colostrum of Murrah buffaloes. 40 newly parturated Murrah buffaloes dams were included in the present study. Polymerase chain reaction- single stranded conformational polymorphism (PCR-SSCP) technique was used to explore the polymorphism in β2M gene of buffaloes. The PCR-SSCP analysis and sequencing of different patterns obtained by silver staining of fragments pertaining to exon 1 through 4 and partial introns 1 through 3, revealed 9 SNPs which were further used to prepare the haplotypes. 7 different haplotypes were observed. Indirect ELISA was done to estimate IgG concentrations in colostrum (collected 1 hour before first milking) obtained from buffaloes. IgG levels in colostrum ranged from 11.22 to 185.1 mg/ml with a mean IgG concentration of 51.71 ± 5.99 mg/ml. The effect of different haplotypes was evaluated on IgG concentrations. The least square analysis of variance could not exhibit a significant effect of buffalo dam haplotype on colostral IgG concentration. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03676722
- Volume :
- 52
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Indian Journal of Animal Research
- Publication Type :
- Academic Journal
- Accession number :
- 127614048
- Full Text :
- https://doi.org/10.18805/ijar.v0iOF.4566