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Turn-on fluorescent assay based on purification system via magnetic separation for highly sensitive probing of adenosine.
- Source :
-
Sensors & Actuators B: Chemical . Apr2018, Vol. 259, p855-861. 7p. - Publication Year :
- 2018
-
Abstract
- In this work, based on purifying the analysis system by magnetic separation, we constructed a turn-on fluorescent assay for highly sensitive detection of possible biomarkers, employing adenosine as the model target analyte. Aptamer labeled-carbon dots (Apt-CDs) was applied as fluorescence probes as well as selective recognition elements, while Fe 3 O 4 @polypyrrole (Fe 3 O 4 @PPY) was used as fluorescence quenching and magnetic separating materials for system purification. In absence of adenosine, the Apt-CDs were attached to the surface of PPY through π-π stacking and hydrophobic interaction and its fluorescence was quenched by Fe 3 O 4 @PPY. In presence of adenosine, the aptamer could specifically bind with adenosine to form a three dimensional Apt-CDs-adenosine complex and detach from PPY, leading to the fluorescence recovery of Apt-CDs. More importantly, with analysis system purification via twice convenient magnetic separations of free CDs and Apt-CDs-adenosine complex respectively, the detection limit could be reduced from 8 nmol L −1 to 0.15 nmol L −1 . This concept offers potential application for simple, rapid, cost-effective, and highly sensitive assay of biological samples. [ABSTRACT FROM AUTHOR]
- Subjects :
- *QUANTUM dots
*FLUOROPHORES
*BIOLUMINESCENCE
*APTAMERS
*NANO-probe sensors
Subjects
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 259
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 127618788
- Full Text :
- https://doi.org/10.1016/j.snb.2017.12.147