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Liraglutide regulates the viability of pancreatic α-cells and pancreatic β-cells through cAMP-PKA signal pathway.
- Source :
-
Life Sciences . Feb2018, Vol. 195, p87-94. 8p. - Publication Year :
- 2018
-
Abstract
- Aims: As a glucagon-like peptide-1 receptor agonist, liraglutide could effectively increase insulin secretion from pancreatic β-cells and suppress glucagon secretion from pancreatic α-cells in the treatment of hyperglycemia in type 2 diabetes patients. However, the mechanisms for the different regulation of pancreatic α-cells and β-cells are still unclear. In this study, we mainly explored the different effects of liraglutide on mouse pancreatic α-cell line and β-cell line in vitro. Main methods: Herein, mouse pancreatic α-cell line, α-TC1-6, and mouse pancreatic β-cell line, β-TC-tet, were used to analyze the biological effects of liraglutide in different concentrations. Cell proliferation, cell apoptosis and cell secretion ability were detected in different groups. Besides, the level of miR-375 and cAMP-PKA signal pathway were further evaluated using qPCR and western blot. Key findings: The results indicated that liraglutide could increase the level of miR-375 and cell apoptosis in pancreatic α-cells through inhibiting the cAMP-PKA signal pathway, but activate cAMP-PKA signal pathway in pancreatic β-cells, and further lead to the down-regulation of miR-375 and improve cell viability. Therefore, the treatment with liraglutide could down-regulate the glucagon secretion ability of α-TC1-6 cells, and the insulin secretion ability of β-TC-tet cells was enhanced with the liraglutide treatment in a dose-dependent manner. Significance: In conclusion, we mainly found that liraglutide could regulate the viability of pancreatic α-cells and pancreatic β-cells through inhibiting and activating cAMP-PKA signal pathway respectively. The better understanding of the mechanism could help us to develop more novel therapy methods for diabetes in the future. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00243205
- Volume :
- 195
- Database :
- Academic Search Index
- Journal :
- Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 127641160
- Full Text :
- https://doi.org/10.1016/j.lfs.2017.12.012