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Frequencies of epitope-specific cytotoxic T lymphocytes in active chronic viral hepatitis B infection by using MHC class I peptide tetramers
- Source :
-
Immunology Letters . Apr2004, Vol. 92 Issue 3, p253. 6p. - Publication Year :
- 2004
-
Abstract
- Hepatitis B virus (HBV)-specific cytotoxic T lymphocytes (CTLs) are thought to play key roles in viral control and liver damage. We have used HLA-A*02 tetramer complex to human HBV core 18–27 (Tc 18–27), envelope 183–191 (Te 183–191), envelope 335–343 (Te 335–343), and polymerase 575–583 (Tp 575–583) epitopes to characterize HLA class I-restricted CD8+ T cells in active chronic HBV infection. The frequencies of specific epitopes circulating tetramer+ cells were determined in whole-blood samples by analysis of flow cytometry. The correlation of HBV epitope-specific CTL, between viral replication and liver damage, was analyzed by multiple regression. Our data shown that HBV-specific CD8+ T cells can be easily detected in peripheral blood of active chronic HBV infections. No significant correlation was found between either the frequency of HBV-specific CD8+ T cells and the viral load, or the frequency of HBV-specific CD8+ T cells and the levels of alanine transaminase. These results suggest that the existence of epitope-specific HBV CTLs are not directly correlated to hepatocyte injury, and the frequencies of HBV-specific T cells are not determinant of immune-mediated protection in chronic HBV infection. [Copyright &y& Elsevier]
- Subjects :
- *HEPATITIS B virus
*T cells
*EPITOPES
*FLOW cytometry
Subjects
Details
- Language :
- English
- ISSN :
- 01652478
- Volume :
- 92
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Immunology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 12778501
- Full Text :
- https://doi.org/10.1016/j.imlet.2004.01.002