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Next-generation sequencing of the cerebrospinal fluid in the diagnosis of neurobrucellosis.

Authors :
Fan, Siyuan
Ren, Haitao
Wei, Yanping
Mao, Chenhui
Ma, Zhenzi
Zhang, Lu
Wang, Li
Ge, Ying
Li, Taisheng
Cui, Liying
Wu, Honglong
Guan, Hongzhi
Source :
International Journal of Infectious Diseases. Feb2018, Vol. 67, p20-24. 5p.
Publication Year :
2018

Abstract

Background Brucellosis is the most common zoonotic infection in the world. Brucellosis with nervous system involvement is known as ‘neurobrucellosis’ (NB). The diagnosis of NB is difficult because its clinical manifestations are non-specific and the sensitivity of routine culture tests is low. Methods Next-generation sequencing (NGS) of cerebrospinal fluid (CSF) was used to detect pathogens in patients with clinically suspected central nervous system (CNS) infections at a tertiary referral center in China between June 1, 2016 and June 1, 2017. The clinical characteristics and NGS results of patients with the diagnosis of NB were reviewed in this study. Results Four patients were rapidly diagnosed with NB using NGS of the CSF in patients with clinically suspected CNS infections, although the clinical manifestations varied dramatically between these patients. NGS of the CSF revealed that the sequence reads identified that corresponded to Brucella species ranged from 11 to 104, with genomic coverage ranging from 0.043% to 0.4%. Rapid diagnosis led to prompt treatment with the appropriate antibiotics. Conclusions This study demonstrates the power of NGS of the CSF coupled with a bioinformatic pipeline in the diagnosis of NB. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
12019712
Volume :
67
Database :
Academic Search Index
Journal :
International Journal of Infectious Diseases
Publication Type :
Academic Journal
Accession number :
127791851
Full Text :
https://doi.org/10.1016/j.ijid.2017.11.028