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Isolation of ellagic acid from pomegranate peel extract by hydrophobic interaction chromatography using graphene oxide grafted cotton fiber adsorbent.
- Source :
-
Journal of Separation Science . Feb2018, Vol. 41 Issue 3, p747-755. 9p. - Publication Year :
- 2018
-
Abstract
- Abstract: Ellagic acid, a natural polyphenol, was isolated from pomegranate peel extract by hydrophobic interaction using graphene oxide grafted cotton fiber as a stationary adsorbent. The grafted graphene oxide moieties served as hydrophobic interaction‐binding sites for ellagic acid adsorption. The graphene oxide grafted cotton fiber was made into a membrane‐like sheet in order to complete ellagic acid purification by using a binding–elution mode. The effects of operational parameters, such as the composition of the binding buffer/elution buffer, buffer pH, and buffer concentration, on the isolation process were investigated. It was found that 5 mmol/L sodium carbonate aqueous solution is a proper‐binding buffer, and sodium hydroxide aqueous solution ranging from 0.04 to 0.06 mol/L is a suitable elution solution for ellagic acid purification. Under the optimized condition, the purity of ellagic acid increased significantly from 7.5% in the crude extract to 75.0–80.0%. The pH value was found to be a key parameter that determines the adsorption and desorption of ellagic acid. No organic solvent is involved in the entire purification process. Thus, a simple and environmentally friendly method is established for ellagic acid purification using a graphene oxide‐modified biodegradable and bio‐sourced fibrous adsorbent. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ELLAGIC acid
*POMEGRANATE
*FRUIT extracts
*HYDROPHOBIC interactions
*GRAPHENE oxide
Subjects
Details
- Language :
- English
- ISSN :
- 16159306
- Volume :
- 41
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Separation Science
- Publication Type :
- Academic Journal
- Accession number :
- 127818924
- Full Text :
- https://doi.org/10.1002/jssc.201700896