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Formation of Malonaldehyde from Phospholipid Arachidonate during Microsomal Lipid Peroxidation.
- Source :
-
European Journal of Biochemistry . 1968, Vol. 6 Issue 1, p126-130. 5p. - Publication Year :
- 1968
-
Abstract
- The thiobarbituric acid reacting material produced during enzymatic microsomal lipid peroxidation has been identified as malonaldehyde. The malonaldehyde was condensed with urea to form 2-hydroxy-pyrimidine, which was identified by its ultraviolet spectrum, chromatographic properties, and mass spectrum. Incubations with phosphatidyl choline labelled with tritiated arachidonate yielded 2-hydroxy-pyrimidine with a specific activity nearly equal to that of the phospholipid arachidonate. Incubations with tritiated arachidonic acid yielded 2-hydroxy-pyrimidine with a specific activity nearly 2 orders of magnitude less than that of free arachidonic acid. Therefore, phospholipid arachidonate has been established as the major source of the malonaldehyde produced during microsomal lipid peroxidation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 6
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12793677
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1968.tb00428.x