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Qualitative and quantitative analysis of catechin and quercetin in flavonoids extracted from Rosa roxburghii Tratt.
- Source :
-
Tropical Journal of Pharmaceutical Research . Jan2018, Vol. 17 Issue 1, p71-76. 6p. - Publication Year :
- 2018
-
Abstract
- Purpose: To perform a qualitative and quantitative analysis of catechin and quercetin in flavonoids extracted from Rosa roxburghii Tratt. Methods: Total flavonoids were determined using ultraviolet spectrophotometry (UV) at 500 nm. The optimal gradient program started with 15 % methanol and was kept within a period of 0 - 20 min, while 25 % methanol was kept within 20 - 33 min. Subsequently, the concentration of methanol was reduced to 15 % and was held for 10 min until the next injection. Mass spectrometry spray voltage was 4,000 V, ionization temperature 350 °C, atomizer pressure 35 psi, nitrogen flow rate 8 L/min, and mass scan range 200 - 800 m/z. The detection wavelength used for catechin and quercetin was 270 and 368 nm, respectively. Results: Based on the UV results, Rosa roxburghii Tratt content was 73.85 %, which is in agreement with the national standard. Liquid chromatography-mass spectrometry (LC-MS) results indicate that Rosa roxburghii Tratt flavonoids contained quercetin, 34.26 %, with relative standard deviation (RSD) of 2.88 % and catechin content of 2.97 % with RSD of 1.49 %. Conclusion: The proposed measurement method for determining the content of flavonoids in Rosa roxburghii Tratt has the advantage of simplicity, feasibility, good repeatability, and rapid and accurate analysis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CATECHIN
*QUERCETIN
*FLAVONOIDS
*PLANT extracts
*ULTRAVIOLET spectrophotometry
Subjects
Details
- Language :
- English
- ISSN :
- 15965996
- Volume :
- 17
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Tropical Journal of Pharmaceutical Research
- Publication Type :
- Academic Journal
- Accession number :
- 128049050
- Full Text :
- https://doi.org/10.4314/tjpr.v17i1.11