An Automated Technique for Canine Blood Typing.

This article presents the study of canine erythrocyte antibodies. An adaptation of the Polybrene technique for typing of canine erythrocytes is reported in this paper. Dog whole blood was anticoagulated with acid-citrate-dextrose in a proportion of 1:9 The samples were stored at 40, and used within 10 days. Manual typing and titrations were performed. Adaptation of this technique to canine blood study required two modifications: 15 per cent bovine serum albumin was substituted for homologous normal serum; the concentration of Polybrene was reduced to 0.033 mg/ml. Results demonstrated that the Polybrene technique is from 12.5 (anti-A) to 3200-fold (anti-He) more sensitive than the manual technique.