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Artesunate enhances γδ T-cell-mediated antitumor activity through augmenting γδ T-cell function and reversing immune escape of HepG2 cells.
- Source :
-
Immunopharmacology & Immunotoxicology . Apr2018, Vol. 40 Issue 2, p107-116. 10p. - Publication Year :
- 2018
-
Abstract
- <bold>Objective:</bold> To explore the effect and mechanism of artesunate on γδ T cell-mediated antitumor immune responses against hepatoma carcinoma cells (HepG2) <italic>in vitro</italic>. <bold>Methods:</bold> Human γδ T cells or HepG2 were respectively treated with artesunate, subjected to co-culture as appropriate, and the following assays were subsequently conducted: CCK8 to examine cell viability; LDH release assay to detect the killing effect of γδ T cells on HepG2 cells; flow cytometry to examine the expression of perforin (PFP) and granzyme B (GraB) of γδ T cells; ELISA to evaluate the levels of TGF-β1 and IL-10 in the collected supernatant of HepG2 cells pretreated with artesunate; and Western blot analysis to examine Fas, FasL, STAT3, p-STAT3 expression of HepG2 cells induced by artesunate. <bold>Results:</bold> The results showed that the cytotoxicity effect of γδ T cells pretreated with artesunate on HepG2 cells was augmented via elevating the expression of GraB in γδ T cells. Furthermore, treatment with artesunate reversed the inhibition of HepG2 cells on γδ T cells by reducing the secretion of TGF-β1 in HepG2 cells supernatant and enhanced the antitumor effect of γδ T cells against HepG2 cells through increasing the expression of Fas on HepG2 cells, which may be attributed to the inhibition of STAT3 signaling protein. <bold>Conclusion:</bold> Artesunate has several mechanisms for augmenting the antitumor immune responses mediated by γδ T cells. These results suggested artesunate may be an efficacious agent in the treatment of hepatocellular carcinoma. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08923973
- Volume :
- 40
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Immunopharmacology & Immunotoxicology
- Publication Type :
- Academic Journal
- Accession number :
- 128460883
- Full Text :
- https://doi.org/10.1080/08923973.2017.1386212