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A water-soluble fluorescence “turn on” chemosensor for Cu2+ signaling: A combined photophysical and cell imaging study.
- Source :
-
Journal of Photochemistry & Photobiology A: Chemistry . Apr2018, Vol. 357, p49-59. 11p. - Publication Year :
- 2018
-
Abstract
- A simple and effective fluorescence “turn on” chemosensor (ZPA) has been synthesized for rapid, sensitive and selective detection of copper ions (Cu 2+ ) in fully aqueous buffer. The binding interaction between ZPA and Cu 2+ was investigated through UV–vis absorption, fluorescence measurement, mass spectroscopy, and IR spectrum analysis. Cu 2+ could specifically bind with the hydroxyl-O and imine-N atom in ZPA with high affinity ( K a = 6.08 × 10 7 M −1 ). Coordination of Cu 2+ to ZPA restricting the photoinduced electron transfer (PET) process in free ZPA and generating a more rigid ZPA/Cu 2+ complex with an extended π-electron conjugation system, sequentially leading to the fluorescence enhancement of ZPA. The detection limit (calculated to be 1.49 × 10 −8 M) was far below the limit in the World Health Organization (WHO) guidelines for drinking water (30 μM) and the sensing process was fully reversible. Practical applications of ZPA for Cu 2+ detection in real water samples were investigated with satisfactory results. Endowed with attributes like high binding affinity, specificity, relatively long excitation/emission properties and excellent bio-compatibility, ZPA demonstrated outstanding fluorescent imaging of intracellular Cu 2+ in living cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CHEMORECEPTORS
*CELL imaging
*COPPER ions
*IMINES
*MASS spectrometry
Subjects
Details
- Language :
- English
- ISSN :
- 10106030
- Volume :
- 357
- Database :
- Academic Search Index
- Journal :
- Journal of Photochemistry & Photobiology A: Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 128517169
- Full Text :
- https://doi.org/10.1016/j.jphotochem.2018.02.020