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An alternative and simple method to consistently prepare viable isolated human islets for clinical transplantation
- Source :
-
Transplantation Proceedings . Apr2004, Vol. 36 Issue 3, p605. 2p. - Publication Year :
- 2004
-
Abstract
- We describe a method to consistently prepare human islets for transplantation. By combining a simple collagenase digestion method and a density gradient purification system, we were able to obtain successful isolations (≥200,000 islet equivalents, ≥50% purity) in 69% of processed glands. No reagent of animal source was used. Isolated islets were morphologically well maintained and functionally competent, with sterility confirmed in 97% of cases. Two patients were transplanted with islets prepared by this method; graft function was demonstrated for a few months. Improved simplicity and consistency, together with adequate quality of the preparations, are the main features of this isolation method. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00411345
- Volume :
- 36
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Transplantation Proceedings
- Publication Type :
- Academic Journal
- Accession number :
- 12899296
- Full Text :
- https://doi.org/10.1016/j.transproceed.2004.02.047