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Sequential gene promoter interactions by C/EBPβ, C/EBPα, and PPARγ during adipogenesis
- Source :
-
Biochemical & Biophysical Research Communications . May2004, Vol. 318 Issue 1, p213. 6p. - Publication Year :
- 2004
-
Abstract
- Treatment of 3T3-L1 preadipocytes with differentiation inducers triggers a cascade in which C/EBPβ is rapidly expressed, followed by C/EBPα and PPARγ. C/EBPα and PPARγ then activate the expression of adipocyte genes that produce the differentiated phenotype. Circumstantial evidence indicates that C/EBPβ activates transcription of the C/EBPα and PPARγ genes, both of which possess C/EBP regulatory elements in their proximal promoters. Although C/EBPβ is expressed immediately upon induction of differentiation, acquisition of DNA binding activity is delayed for ∼14 h. Chromatin immunoprecipitation (ChIP) analysis conducted 24 h after induction revealed that C/EBPβ binds to C/EBP regulatory elements in the proximal promoters of the C/EBPα and PPARγ genes. ChIP analysis showed that after an additional delay C/EBPα binds to its own promoter and to the promoters of the PPARγ and 422/aP2 genes. These findings support the view that once expressed, C/EBPα is responsible for maintaining the expression of PPARγ, and C/EBPα, as well as adipocyte proteins (e.g., 422/aP2) in the terminally differentiated state. Together these findings provide compelling evidence that C/EBPβ, C/EBPα, and PPARγ participate in a cascade during adipogenesis. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 318
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 12899605
- Full Text :
- https://doi.org/10.1016/j.bbrc.2004.04.017