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The influence of fatty acid supply and aldehyde reductase deletion on cyanobacteria alkane generating pathway in <italic>Escherichia coli</italic>.
- Source :
-
Journal of Industrial Microbiology & Biotechnology . May2018, Vol. 45 Issue 5, p329-334. 6p. 3 Graphs. - Publication Year :
- 2018
-
Abstract
- Cyanobacteria alkane synthetic pathway has been heterologously constructed in many microbial hosts. It is by far the most studied and reliable alkane generating pathway. Aldehyde deformylating oxygenase (i.e., ADO, key enzyme in this pathway) obtained from different cyanobacteria species showed diverse catalytic abilities. This work indicated that single aldehyde reductase deletions were beneficial to <italic>Nostoc punctiforme</italic> ADO-depended alkane production in <italic>Escherichia coli</italic> even better than double deletions. Fatty acid metabolism regulator (FadR) overexpression and low temperature increased C18:1 fatty acid supply, and in turn stimulated C18:1-derived heptadecene production, suggesting that supplying ADO with preferred substrate was important to overall alkane yield improvement. Using combinational methods, 1 g/L alkane was obtained in fed-batch fermentation with heptadecene accounting for nearly 84% of total alkane. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13675435
- Volume :
- 45
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Industrial Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 129209219
- Full Text :
- https://doi.org/10.1007/s10295-018-2032-6