A multifunctional force microscope for soft matter with <italic>in situ</italic> imaging.

We present the multifunctional force microscope (MFM), a normal and lateral force-measuring instrument with <italic>in situ</italic> imaging. In the MFM, forces are calculated from the normal and lateral deflection of a cantilever as measured via fiber optic sensors. The motion of the cantilever is controlled normally by a linear micro-translation stage and a piezoelectric actuator, while the lateral motion of the sample is controlled by another linear micro-translation stage. The micro-translation stages allow for travel distances that span 25 mm with a minimum step size of 50 nm, while the piezo has a minimum step size of 0.2 nm, but a 100 <italic>μ</italic>m maximum range. Custom-designed cantilevers allow for the forces to be measured over 4 orders of magnitude (from 50 <italic>μ</italic>N to 1 N). We perform probe tack, friction, and hydrodynamic drainage experiments to demonstrate the sensitivity, versatility, and measurable force range of the instrument. [ABSTRACT FROM AUTHOR]