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Localization Microscopy of Actin Cytoskeleton in Human Platelets.
- Source :
-
International Journal of Molecular Sciences . Apr2018, Vol. 19 Issue 4, p1150. 10p. 1 Color Photograph, 1 Black and White Photograph, 1 Graph. - Publication Year :
- 2018
-
Abstract
- Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional accuracy of 12 nm. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CYTOSKELETON
*PHALLOIDINE
*ACTIN
*RHODAMINES
*FLUOROPHORES
Subjects
Details
- Language :
- English
- ISSN :
- 16616596
- Volume :
- 19
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- International Journal of Molecular Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 129456174
- Full Text :
- https://doi.org/10.3390/ijms19041150