大鼠缺血性脑卒中脑皮层蛋白质组学分析.

Objective To explore brain cortex proteomics changes during ischemic cerebral stroke (ICS), and to seek the biomarkers associated with ICS. Methods A total of 42 male SD rats were randomly divided into sham group (n=12) and ICS group (n=30). ICS model was induced by distal middle cerebral artery occlusion. Brain cortex tissues were collected at 1,6,24 and 48 h after ICS, respectively, after cerebral ischemia. Samples from different groups were subjected to tandem mass tag (TMT)-based quantitative proteomics analysis for identification of differentially expressed proteins. Gene Ontology enrichment analysis was utilized to classify the differentially expressed proteins. Western blot was used to verify the quantitative proteomics results. Results Compared with sham group, 25 proteins were considered to be differentially expressed in ICS group. Gene Ontology enrichment analysis revealed that the differentially expressed proteins were related to acute-phase response, inflammatory response, lipoprotein metabolic process, complement activation, classical pathway and innate immune response. The expression change of heat shock protein 27 (HSP27) was basically consistent with the quantitative proteomics results. Conclusions These 25 differentially expressed proteins may serve as potential biomarkers for ICS. [ABSTRACT FROM AUTHOR]