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d-Tagatose manufacture through bio-oxidation of galactitol derived from waste xylose mother liquor.
- Source :
-
Green Chemistry . 5/21/2018, Vol. 20 Issue 10, p2382-2391. 10p. - Publication Year :
- 2018
-
Abstract
- In this study, the bio-oxidation of galactitol to the valuable d-tagatose is presented. This proposed strategy could start with the refined by-product of waste xylose mother liquor formed by chemical hydrogenation. Through a computationally guided enzyme screening approach, a robust polyol dehydrogenase (PdPDH) was rapidly identified from a massive number of candidates. When coupled with a water-forming NADH oxidase (StNOX) for environmentally benign cofactor regeneration, subsequent reaction optimization facilitated the complete transformation of 100 g L−1 galactitol into the desired product within 15 h, with a yield of 91% in a 2 L scale preparative reaction. Compared to the current enzymatic isomerization system, our approach avoids low conversion, high operative temperatures and by-product formation, while enabling simplified product isolation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14639262
- Volume :
- 20
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Green Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 129710619
- Full Text :
- https://doi.org/10.1039/c8gc00091c