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Rapid and universal detection of ovalbumin based on N,O,P-co-doped carbon dots-fluorescence resonance energy transfer technology.
- Source :
-
Sensors & Actuators B: Chemical . Sep2018, Vol. 269, p278-287. 10p. - Publication Year :
- 2018
-
Abstract
- Fluorescence resonance energy transfer (FRET), a near-field energy transfer from a fluorescent donor to a fluorescent acceptor within close proximity, has been widely used in biosensing. In this study, a rapid and universal sensor for the detection of ovalbumin (OVA) using nitrogen, oxygen, phosphoric co-doped CDs (NOP-CDs) and graphene oxide (GO) was established based on the FRET technology. First, novel NOP-CDs peak at 443 nm were synthesized by a one-pot hydrothermal method using egg powder and phosphorus acid. Then, in the absence of OVA, the fluorescence of OVA-antibody modified NOP-CDs was quenched by GO via electrostatic attraction and π-π stacking interaction due to the FRET effect. The introduced OVA competed to displace the GO by special binding with the anti-OVA, resulting in the recovery of the fluorescence. This sensor displayed a linear response to OVA from 0.5 to 15 μg/mL with a detection limit of 153 ng/mL and was successfully applied to the detection of the OVA sample with recoveries ranging from 99.25 to 118.90%. In summary, the green and easy-to-synthesize nano-material was used in this method, the accurate detection results, and the simple process exhibited the great potential of this method for practical applications. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 269
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 129790585
- Full Text :
- https://doi.org/10.1016/j.snb.2018.04.134