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Total Flavones of Rhododendron simsii Planch Flower Protect against Cerebral Ischemia-Reperfusion Injury via the Mechanism of Cystathionine-γ-Lyase-Produced H2S.
- Source :
-
Evidence-based Complementary & Alternative Medicine (eCAM) . 5/31/2018, Vol. 2018, p1-11. 11p. 7 Graphs. - Publication Year :
- 2018
-
Abstract
- Total flavones of Rhododendron simsii Planch flower (TFR) have a significant protective effect against cerebral ischemia-reperfusion injury. However, its mechanism is unclear. This study investigated the protection of TFR against cerebral ischemia-reperfusion injury via cystathionine-γ-lyase- (CSE-) produced H2S mechanism. CSE-/- mice and CSE-siRNA-transfected rat were used. Relaxation of cerebral basilar artery (CBA), H2S, and CSE mRNA were measured. TFR significantly inhibited cerebral ischemia-reperfusion-induced abnormal neurological symptom and cerebral infarct in the normal rats and the CSE+/+ mice, but not in the CSE-/- mice, and the inhibition was markedly attenuated in CSE-siRNA-transfected rat; TFR elicited a significant vasorelaxation in rat CBA, and the relaxation was markedly attenuated by removal of endothelium or CSE-siRNA transfection or coapplication of NO synthase inhibitor L-NAME and PGI2 synthase inhibitor Indo. CSE inhibitor PPG drastically inhibited TFR-evoked vasodilatation resistant to L-NAME and Indo in endothelium-intact rat CBA. TFR significantly increased CSE mRNA expression in rat CBA endothelial cells and H2S production in rat endothelium-intact CBA. The increase of H2S production resistant to L-NAME and Indo was abolished by PPG. Our data indicate that TFR has a protective effect against the cerebral ischemia-reperfusion injury via CSE-produced H2S and endothelial NO and/or PGI2 to relax the cerebral artery. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CEREBRAL artery physiology
*CEREBRAL ischemia
*THERAPEUTICS
*REPERFUSION injury
*BASILAR artery
*ANIMAL experimentation
*ARGININE
*VASODILATION
*EPITHELIAL cells
*FLOWERS
*GENE expression
*GENETIC techniques
*HYDROGEN sulfide
*INFARCTION
*LYASES
*MEDICINAL plants
*MICE
*NERVOUS system
*PROSTACYCLIN
*RATS
*RNA
*FLAVONES
*PHARMACODYNAMICS
*PHYSIOLOGY
*PREVENTION
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Volume :
- 2018
- Database :
- Academic Search Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 129883942
- Full Text :
- https://doi.org/10.1155/2018/8903849