细胞膜离子通道电流检测仿真实验教学设备研制.

Patch-clamp amplifier is used to measure the ion channel transmembrane currents generated by charged ions passing through the cell membrane, the equipment is costly. The application of patch clamp ion channel measurement in undergraduate teaching is restricted by the teaching budget. The computer simulation technique was used to simulate the experimental operating environment, and the measurement of the sealing resistance detection circuit was carried out. The hardware system of the instrument adopted the movement of the negative pressure syringe to drive the resistance variable of the sealing process. The software system used MSP430 monitoring software and microcomputer display analysis and processing software. Finally, the experimental equipment of ion channel current simulation of cell membrane was developed. The application of this instrument achieved the purpose of conducting experimental teaching of ion channel current measurement, and better promoted the students to master cell membrane ion channel current detection technology. [ABSTRACT FROM AUTHOR]