Barr bodies in pulpal tissue as a diagnostic tool for gender determination.

Background: Gender can also be determined by the study of X and Y chromosomes in the cells which are not undergoing active division. The presence or absence of X chromosome can be studied from buccal smears, skin biopsy, blood, cartilage, hair root sheath, and tooth pulp. After death, it persists for variable periods depending on the humidity and temperature in which tissue has remained. X chromatin and intranuclear structure are also known as Barr body. Aim: The aim of this study is to evaluate the diagnostic performance of X Barr body and Y Barr body observed in pulp tissue as a tool for gender determination. Materials and Methods: The research was conducted in 30 patients (15 male and 15 female). The tooth extracted was from the anteriors of maxilla and mandible. The removed pulpal tissue is stored in 5% formalin for 7 days, paraffin blocks are prepared and the block is cut at 5 µm thickness and stained with hematoxylin and eosin and is viewed under a trinocular microscope under ×100 magnification, and 50 cells are viewed for Barr bodies. Result: In females, the mean of Barr bodies was found to be 13 ± 2, and in the males, it was found to be 5 ± 3. Conclusion: Thus, it can be concluded that the pulp tissues with a maximum number of Barr bodies belong to a female and pulp with a minimum number of Barr bodies belongs to a male. [ABSTRACT FROM AUTHOR]