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Low reliability of anti-KIR4.183–120 peptide auto-antibodies in multiple sclerosis patients.
- Source :
-
Multiple Sclerosis Journal . Jun2018, Vol. 24 Issue 7, p910-918. 9p. 2 Charts, 4 Graphs. - Publication Year :
- 2018
-
Abstract
- Background: Multiple sclerosis (MS) is an autoimmune disease for which auto-antibodies fully validated as diagnostic and prognostic biomarkers are widely desired. Recently, an immunoreactivity against the inward rectifying potassium channel 4.1 (KIR4.1) has been reported in a large proportion of a group of MS patients, with amino acids 83–120 being the major epitope. Moreover, a strong correlation between anti-KIR4.183–120 and anti-full-length-protein auto-antibodies titer was reported. However, this finding received limited confirmation. Objective: Validation of the diagnostic potential of anti-KIR4.183–120 antibodies in 78 MS patients, 64 healthy blood donors, and 42 individuals with other neurological diseases. Methods: Analysis of anti-KIR4.183–120 antibodies by enzyme-linked immunosorbent assay (ELISA) using a mouse antiserum we produced as a new ELISA reliability control. Additionally, evaluation of reactivity against 293-T cells transiently transfected with full-length KIR4.1 by flow cytometry. Results: We found antibodies to KIR4.183–120 only in 13 out of 78 (16.6%) MS patients; among these, only 2 were positive for anti-full-length KIR4.1 antibodies. Conclusion: Employing a new reliability control and a new cytofluorometric assay, we cannot support anti-KIR4.183–120 auto-antibodies as a reliable biomarker in MS. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13524585
- Volume :
- 24
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Multiple Sclerosis Journal
- Publication Type :
- Academic Journal
- Accession number :
- 130228594
- Full Text :
- https://doi.org/10.1177/1352458517711275