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A novel fluorescence “turn off-on” nano-sensor for detecting Cu2+ and Cysteine in living cells.
- Source :
-
Journal of Photochemistry & Photobiology A: Chemistry . Jul2018, Vol. 362, p14-20. 7p. - Publication Year :
- 2018
-
Abstract
- In this work, a novel fluorescent sensor (quercetin-Pluronic-F127 nano-micelles) was prepared by the facile self-assembly method with natural quercetin (Qc) and Pluronic-F127 (F127). Qc-F127 nano-micelles were characterized by TEM which indicated that Qc have successfully entered into the amphiphilic micelle. The nano micelles with sizes of 25 to 40 nm showed a strong green fluorescence (FL). The sensor exhibits high sensitivity and selectivity towards Cu 2+ with a linear range of 5 × 10 −8 –4 × 10 −6 mol L −1 , the detection limit of 1 × 10 −8 mol L −1 . Moreover, the Qc-F127 nano-micelles can be used to detect Cu 2+ in vitro due to their good biocompatibility. Besides Cu 2+ sensing, when Cysteine (Cys) were added, the FL intensity of Qc-F127-Cu(II) system could be restored due to the chelation interactions between Cu 2+ ions and the sulfydryl and amino groups of Cys. So, Qc-F127-Cu(II) system can used to detect Cys, and the linear range and the limit of detection of the Qc-F127-Cu 2+ system were 3 × 10 −7 –3.5 × 10 −5 mol L −1 and 6 × 10 −8 mol L −1 . Moreover, Qc-F127-Cu(II) has been used for imaging of Cys in cells, which demonstrates its values for potential application in biological systems. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NANOSENSORS
*FLUORESCENCE
*CYSTEINE
*COPPER ions
*MOLECULAR self-assembly
*MICELLES
Subjects
Details
- Language :
- English
- ISSN :
- 10106030
- Volume :
- 362
- Database :
- Academic Search Index
- Journal :
- Journal of Photochemistry & Photobiology A: Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 130302085
- Full Text :
- https://doi.org/10.1016/j.jphotochem.2018.05.004