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Structural characterization and α-glucosidase inhibitory activity of polysaccharides extracted from Chinese traditional medicine Huidouba.
- Source :
-
International Journal of Biological Macromolecules . Oct2018, Vol. 117, p815-819. 5p. - Publication Year :
- 2018
-
Abstract
- In this research, the crude polysaccharides were extracted from Chinese traditional medicine Huidouba and then isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatographic columns. The hypoglycemic activities of two fractions were determined by α-glucosidase inhibitory activity in vitro . The fraction HDBP-3A showed the best α-glucosidase inhibitory activity with an IC 50 of 0.0075 mg/mL, which was lower than the acarbose positive control (IC 50 of 0.092 mg/mL). The structural features of HDBP-3A was investigated by the high-performance gel permeation chromatography (HPGPC), gas chromatography(GC), UV spectroscopy, FT-IR, periodate oxidation and Smith degradation. The results demonstrated that the HDBP-3A with a molecular weight of 86,749 Da mainly composed of l -rhamnose, d -fucose, l -arabinose, d -xylose, d -mannose, d -glucose and d -galactose with the molar ratio of 2.33:1.58:1.00:1.37:4.86:4.00:7.30. The branches of HDBP-3A were composed of (1 → 4)-linked, (1 → 6)-linked, (1 → 2)-linked or (1 → 3)-linked. The IR spectrum implied that β-pyranoside linkage and α-configurations existed in HDBP-3A simultaneously. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 117
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 131030779
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2018.05.192