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Identification of Stable and Enantioselective Lipases for Biotechnological Applications.
- Source :
-
Croatica Chemica Acta . Jul2018, Vol. 91 Issue 2, p249-253. 5p. - Publication Year :
- 2018
-
Abstract
- The implementation of biocatalytic reactions in biotechnological processes requires enzymes with suitable properties. Thus, optimization of existing or identification of novel enzymes is needed. For enzyme optimization, various mutagenesis approaches are used yielding libraries with a large number of protein variants. Additionally, novel enzymes can be identified from metagenomic libraries, which contain bacterial clones expressing DNA isolated from different habitats. Activity-based screening of such libraries is needed to identify enzyme candidates of interest. Here, we provide a protocol for screening of regio- and enantioselective lipases stable under industrially relevant conditions. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00111643
- Volume :
- 91
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Croatica Chemica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 131035695
- Full Text :
- https://doi.org/10.5562/cca3357