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Developmental characterization and environmental stress responses of Y-box binding protein 1 gene (AccYB-1) from Apis cerana cerana.
- Source :
-
Gene . Oct2018, Vol. 674, p37-48. 12p. - Publication Year :
- 2018
-
Abstract
- Y-box binding protein 1 (YB-1) is a member of the cold shock domain protein superfamily and is involved in development, environmental stresses and DNA oxidative damage in many organisms. However, the precise functions of YB-1 are still not well understood in various insects, including bees. In the current study, we identified a YB-1 gene in Apis cerana cerana ( AccYB-1 ). The predicted cis-acting elements in the promoter sequence of AccYB-1 indicated its possible roles in development and stress responses. AccYB-1 expression was higher in one-day-old larvae and dark-eyed pupae than in other development stages. Tissue-specific expression analysis showed that the mRNA level of AccYB-1 was higher in the thorax and midgut than in other tissues. The results from real-time PCR showed that AccYB-1 was induced by many environmental stresses. Silencing AccYB-1 downregulated the transcriptional level of some growth- and development-related genes and antioxidant genes and decreased the enzyme activities of several antioxidant-related enzymes, further indicating a possible function of AccYB-1 in growth, development and stress responses. Taken together, our findings suggest that AccYB-1 may play an indispensable role in growth and development and environmental stress responses in Apis cerana cerana . To our knowledge, this is the first paper to explore the role of YB-1 in bees. [ABSTRACT FROM AUTHOR]
- Subjects :
- *COLD shock proteins
*DNA
*INSECTS
*STRESS management
*LARVAE
*TISSUES
*GENES
*PUPAE
Subjects
Details
- Language :
- English
- ISSN :
- 03781119
- Volume :
- 674
- Database :
- Academic Search Index
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 131129395
- Full Text :
- https://doi.org/10.1016/j.gene.2018.06.071