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Impact of steroid hormones E2 and P on the NLRP3/ASC/Casp1 axis in primary mouse astroglia and BV-2 cells after in vitro hypoxia.
- Source :
-
Journal of Steroid Biochemistry & Molecular Biology . Oct2018, Vol. 183, p18-26. 9p. - Publication Year :
- 2018
-
Abstract
- Highlights • Hypoxia impacts on the inflammasome NLRP3/ASC/caspase-1 axis in BV-2 cells and primary astroglia. • Estrogen and progesterone counteracts hypoxia-induced effects on NLRP3 and ASC. • Sex steroids fail to mitigate caspase-1 mediated IL1beta maturation. Abstract Clinical and animal model studies have demonstrated the neuroprotective and anti-inflammatory effects of 17beta-estradiol (E2) and progesterone (P) in different disease models of the central nervous system (CNS) including ischemic stroke. Inflammasomes are involved in the interleukin-1 beta (IL1beta) maturation, in particular, NLRP3, the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC) and the active caspase-1 (Casp1) form. Recently, we showed that administration of E2 or P selectively regulated these components after experimental ischemic stroke in rats. Therefore, we investigated the impact of E2 and P on the NLRP3/ASC/Casp1 axis in the murine microglia-like cell line BV-2 cells and primary astrocytes after short-term in vitro hypoxia. The inflammatory cytokine IL1beta but not IL18 was increased after short-term hypoxia in astroglia and BV-2 cells. The same applied to NLPR3 and ASC. Casp1 activity was also elevated in astroglia and BV-2 cells after hypoxia. The administration of E2 or P selectively dampened IL1beta, ASC and NLRP3 expression mainly in BV-2 cells. Both steroid hormones failed to reduce Casp1 activity after hypoxia. We conclude that E2- and P-mediated anti-inflammatory mechanisms occur upstream of Casp1 through the regulation of NLRP3 and its adaptor ASC. [ABSTRACT FROM AUTHOR]
- Subjects :
- *STEROID hormones
*ASTROCYTES
*HYPOXEMIA
*INFLAMMASOMES
*PROGESTERONE
Subjects
Details
- Language :
- English
- ISSN :
- 09600760
- Volume :
- 183
- Database :
- Academic Search Index
- Journal :
- Journal of Steroid Biochemistry & Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 131559684
- Full Text :
- https://doi.org/10.1016/j.jsbmb.2018.05.003