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Detection of BRAF V600E Mutations With Next-Generation Sequencing in Infarcted Thyroid Carcinomas After Fine-Needle Aspiration.
- Source :
-
American Journal of Clinical Pathology . Aug2018, Vol. 150 Issue 2, p177-185. 9p. 2 Diagrams, 3 Charts. - Publication Year :
- 2018
-
Abstract
- <bold>Objectives: </bold>Fine-needle aspiration (FNA) of thyroid lesions may result in infarction and diagnostic difficulties on subsequent thyroidectomy specimens. Next-generation sequencing (NGS) methods for detection of hallmark driver BRAF V600E mutations may help characterize such tumors in which histologic alterations preclude definitive tissue diagnosis.<bold>Methods: </bold>Thyroidectomy specimens with both malignant FNA diagnoses and resultant infarction were identified from our institutional database. NGS methods were used to detect BRAF V600E mutations in the infarcted thyroid carcinomas.<bold>Results: </bold>Nine thyroid carcinomas with infarction were characterized as BRAF-like papillary thyroid carcinoma based on molecular driver categorization and histologic diagnosis. BRAF V600E mutations were detected in the infarcted tissue in four (67%) of six lesions.<bold>Conclusions: </bold>We demonstrate detection of hallmark BRAF V600E mutations by NGS within infarcted tissue of thyroid carcinomas after FNA. This suggests a potential ancillary method of characterizing infarcted thyroid carcinomas whose altered histology may be nondiagnostic. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NEEDLE biopsy
*GENETIC mutation
THYROID cancer diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 00029173
- Volume :
- 150
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- American Journal of Clinical Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 131850074
- Full Text :
- https://doi.org/10.1093/ajcp/aqy045