miR-454-3p suppresses cell migration and invasion by targeting CPEB1 in human glioblastoma.

MicroRNAs (miRNA/miRs) serve crucial roles in the progression of human glioblastoma (GBM); however, the exact regulatory mechanisms of miRNAs in human GBM remain unclear. The present study aimed to investigate the roles of miR-454-3p in human GBM. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis was performed to examine the expression of miR-454-3p in glioma tissues and adjacent tissues. Human GBM cell lines (LN-229, A172 and GL15) and a normal human astrocyte cells (HA1800) were used for analysis. In addition, RT-qPCR and western blotting were applied for mRNA and protein expression analysis, respectively. The cell proliferation was measured using a Cell Counting kit-8 assay. Furthermore, scratch and Transwell assays were employed for the analysis of cell migration and invasion. A luciferase reporter assay was used to verify the target of miR-454-3p. The results revealed that miR-454-3p was downregulated in the glioma tissues and GBM cell lines, including LN-229, A172 and GL15. Additionally, the overexpression of miR-454-3p significantly suppressed the proliferation, migration and invasion of LN-229 cells. Furthermore, cytoplasmic polyadenylation element-binding protein 1 (CPEB1) was confirmed as a direct target of miR-454-3p. These findings indicated that the overexpression of miR-454-3p inhibited cell proliferation, migration and invasion by downregulating CPEB1. Therefore, miR-454-3p may act as a tumor suppressor and represent an effective therapeutic strategy in GBM. [ABSTRACT FROM AUTHOR]