Back to Search
Start Over
Preparation and evaluation of liposomal clarithromycin with antimicrobial effect.
- Source :
-
Journal of Chinese Pharmaceutical Sciences . Jun2018, Vol. 27 Issue 6, p408-414. 7p. - Publication Year :
- 2018
-
Abstract
- In this study, we developed a novel liposomal delivery system modified by Tat peptide and wheat germ agglutinin (WGA) with antimicrobial effect. Physicochemical parameters, in vitro antimicrobial, time-kill study, cellular uptake, biofilm formation inhibition and in vivo antibacterial efficacy against Methicillin-resistant Staphylococcus aureus (MRSA) were investigated. Minimum inhibitory concentrations (MICs) and colony-forming units (CFUs) in the time-kill study for Tat-WGA-modified liposomal clarithromycin (CLA-TatWGALip) were lower than those of free and other modified liposomal CLA. Flow cytometry analysis disclosed that TatWGALip delivered more coumarin 6 into bacteria. Furthermore, Tat-WGA-modified liposomal CLA efficiently inhibited the formation of MRSA biofiom. CFU of MRSA in the abscess of mice treated with CLA-TatWGALip was significantly lower than that of any others (P<0.01). Collectively, liposomal delivery system modified by Tat and WGA could be a promising anti-resistant infection strategy. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10031057
- Volume :
- 27
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of Chinese Pharmaceutical Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 131958420
- Full Text :
- https://doi.org/10.5246/jcps.2018.06.041