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Preparation and evaluation of liposomal clarithromycin with antimicrobial effect.

Authors :
Wenxi Zhang
Guanghua Peng
Maoyuan Song
Jiaxing Wang
Mengya Yin
Jiajia Li
Yajie Liu
Yuanyuan Zhang
Xinru Li
Source :
Journal of Chinese Pharmaceutical Sciences. Jun2018, Vol. 27 Issue 6, p408-414. 7p.
Publication Year :
2018

Abstract

In this study, we developed a novel liposomal delivery system modified by Tat peptide and wheat germ agglutinin (WGA) with antimicrobial effect. Physicochemical parameters, in vitro antimicrobial, time-kill study, cellular uptake, biofilm formation inhibition and in vivo antibacterial efficacy against Methicillin-resistant Staphylococcus aureus (MRSA) were investigated. Minimum inhibitory concentrations (MICs) and colony-forming units (CFUs) in the time-kill study for Tat-WGA-modified liposomal clarithromycin (CLA-TatWGALip) were lower than those of free and other modified liposomal CLA. Flow cytometry analysis disclosed that TatWGALip delivered more coumarin 6 into bacteria. Furthermore, Tat-WGA-modified liposomal CLA efficiently inhibited the formation of MRSA biofiom. CFU of MRSA in the abscess of mice treated with CLA-TatWGALip was significantly lower than that of any others (P<0.01). Collectively, liposomal delivery system modified by Tat and WGA could be a promising anti-resistant infection strategy. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10031057
Volume :
27
Issue :
6
Database :
Academic Search Index
Journal :
Journal of Chinese Pharmaceutical Sciences
Publication Type :
Academic Journal
Accession number :
131958420
Full Text :
https://doi.org/10.5246/jcps.2018.06.041