稳恒直流电场对鸡胚绒毛尿囊膜血管生长的影响.

BACKGROUND: Tissue-engineered bone has become an important means of repairing maxillofacial bone defects due to its minimally invasive, safe and accurate characteristics. OBJECTIVE: The model of chick chorioallantoic membrane was loaded with different intensities of electric field, to detect the blood vessel growth-related parameters, and to explore the effect of electric field on force-vascularization of tissue-engineered bone, in order to search for a method that can promote force-vascularization of tissue-engineered bone, thus increasing the repair success rate. METHODS: The model of chick chorioallantoic membrane (Inshell model) was established using the chicken embryos hatched for 8 days. The models were randomized into A, B, C, D, and E groups, followed by loaded with 0, -0.75, -1.5, -3 and +1.5 V and steady direct current electric fields at different directions. The images were taken at 0, 12, 24, 36 and 48 hours. The average blood vessel length and growth angle were measured by Image J, and analyzed on SPSS 22.0 software. RESULTS AND CONCLUSION: After loaded with different currents of electric fields, the average growth rate of vessel length in the groups A, B and C was 0.012, 0.023, and 0.047 5 mm/h, respectively. Independent sample t test results revealed that after loaded with -1.5 and 0 V for 48 hours, the average length of blood vessel was (4.22 ±0.436) and (3.21±0.31) mm/mm2, respectively, and the difference was significant (P < 0.01). After loaded with -1.5 and 0 V for 48 hours, the angle of blood vessels was (21.36±20.16)° and (82.17±50.44)°, respectively, and the difference was significant (P < 0.05). The chick chorioallantoic membrane around the electrode in the groups D and E degenerated and chick embryos died. These findings indicate that appropriate negative electricity promotes the growth of blood vessels, and changes the angle of blood vessel growth, suggesting that steady direct current electric field greatly affects chick chorioallantoic membrane. [ABSTRACT FROM AUTHOR]