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MiR-101 inhibits cell proliferation and invasion of pancreatic cancer through targeting STMN1.
- Source :
-
Cancer Biomarkers . 2018, Vol. 23 Issue 2, p301-309. 9p. - Publication Year :
- 2018
-
Abstract
- MiRNAs regulated most genes expression, which were proved important in various tumors. In this study, we want to investigate miR-101 effect and molecular mechanism on pancreatic cancer (PC), the research about this was blank now. RT-PCR analysis showed that miR-101 expression was declined in PC. MTT assay found that miR-101 mimic suppressed cell viability, while suppressing miR-101 facilitated cell proliferation. Transwell assay showed that miR-101 mimic inhibited cell invasion, but promoted cell invasion by miR-101 inhibitor. With TargetScanHuman's help, we verified STMN1 as a specific target of miR-101 and luciferase reporter assay was carried out to further confirm this discovery. STMN1 expression was reduced by miR-101 mimic and increased by miR-101 inhibitor. We next found that STMN1 was elevated in PC and its expression was negatively correlated with miR-101 expression. Furthermore, STMN1 siRNA curbed cell proliferation and invasion, which was opposite to miR-101 inhibitor effect on PC progression and STMN1 siRNA attenuated miR-101 inhibitor effect on cell proliferation and invasion. In conclusion, miR-101 inhibited PC cell proliferation and invasion via regulating STMN1, which provided a potential therapeutic for PC patients. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15740153
- Volume :
- 23
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Cancer Biomarkers
- Publication Type :
- Academic Journal
- Accession number :
- 132660922
- Full Text :
- https://doi.org/10.3233/CBM-181675